Chlamydospore Formation of Candida albicans Using Lyophilized Eicchornia crassipes (Water Hyacinth) As An Alternative Fungal Culture Medium
Keywords:
Candida albicans, chlamydospore, Eicchornia crassipes, fungal culture medium, water hyacinthAbstract
The study evaluates the potential of Water Hyacinth Agar with Polysorbate 80 as a fungal culture medium to stimulate chlamydospore formation of Candida albicans. A total of 104 plates were prepared, with 52 plates for the Water Hyacinth Agar as the experimental group and 52 for the Corn Meal Agar as a positive control group. Clinical isolates of Candida albicans were streaked onto the plates, incubated at 37°C, and viewed microscopically for the presence of chlamydospores after 24, 48, and 72 hours of incubation. The number of chlamydospores were then counted under the low power objective by three readers. For both groups, the median chlamydospore count increased as the incubation time increased, with the highest median chlamydospore count recorded after 72 hours of incubation. At 24, 48, and 72 hours, the median chlamydospore count of Candida albicans was significantly higher in the Corn Meal Agar group compared to that in the Water Hyacinth Agar group because of the higher carbohydrate content in the former. Thus, the presence of the chlamydospores of Candida albicans in Water Hyacinth Agar showed that it has the potential to be an alternative fungal culture medium for chlamydospore formation of Candida albicans.
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