Antihypertensive Effect of Raphanus sativus (Radish) Taproot Ethanol Extract on Albino Mus musculus with Induced Hypertension
Keywords:
Antihypertensive effect, radish taproot ethanol extract, Raphanus sativus taproot, Mus musculus with induced hypertensionAbstract
This study aims to determine the antihypertensive effect of Raphanus sativus (radish) taproot ethanol extract on albino Mus musculus (mouse) with induced hypertension. Antihypertensive effect was defined as a decrease of 3% and 4% in systolic blood pressure (SBP) and diastolic blood pressure (DBP), respectively. A randomized controlled trial experimental procedure was conducted with a total of 36 albino mice as subjects, with 12 mice in each treatment group (experimental, positive control, and negative control groups). The Non-invasive Blood Pressure Recorder Series 5800 and Lab Chart ver. 8 software were used to measure and record blood pressure. The baseline blood pressure was recorded in the first week. Hypertension was induced using fructose-NaCl solution as drinking water ad libitum for 7 days, with blood pressure recordings performed thereafter. The powdered plant material underwent soxhlation to extract the Raphanus sativus (radish) taproot ethanol extract. Treatment was administered for 7 days via oral gavage, with blood pressure recordings performed thereafter. Post-treatment SBP and DBP of the positive control group were within the normotensive range (Pre-treatment SBP: 202.20±14.71 mmHg, DBP: 154.48±24.47 mmHg; post-treatment SBP: 137.16±7.82 mmHg, DBP: 93.69±18.96 mmHg) while those in the experimental group were above the normotensive range (Pre-treatment SBP: 200.80±13.95 mmHg, DBP: 159.52±22.68 mmHg; post-treatment SBP: 172.79±11.91 mmHg, DBP: 127.28±11.00 mmHg) but there was a significant decrease in their SBP and DBP. Lastly, the negative control showed no decrease in SBP and DBP (Pre-treatment SBP: 206.47±19.66 mmHg, DBP: 162.75±22.23 mmHg; post-treatment SBP: 214.69±18.36 mmHg, DBP: 160.06±30.44 mmHg). Raphanus sativus taproot ethanol extract had a potential antihypertensive effect since there was at least a 3% decrease in systolic blood pressure and at least a 4% decrease in the diastolic blood pressure in the albino mice with induced hypertension. Further exploration and testing of the antihypertensive effect could be done in future studies through increasing the duration of treatment and performance of toxicity testing. Extract purification technique is also recommended to have a greater yield of the active component of the extract and the use of genetically hypertensive mice for a more homogenous baseline value.
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Copyright (c) 2023 Teresita E. Chiu, Angelo Miguel Ballesteros, Jude Oliver Aves, Edward Bongalos, Dianne Dente, Mahender Devaram Choudhary, Phrexus Docena, Godan Longakit, Zipporah Keim Ortega, Carolly Tabaloc, Raphaella Kailtim
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