In Vitro Anticoagulant Activity of Zingiber officinale (Ginger) Leaf Extract on Human Whole Blood Using Prothrombin Time and Activated Partial Thromboplastin Time
Keywords:
anticoagulant activity, human whole blood, partial thromboplastin time, prothrombin time, Zingiber officinale (Ginger) crude leaf extractAbstract
This study aims to determine the in vitro anticoagulant activity of Zingiber officinale (Ginger) crude leaf extract on human whole blood. Prothrombin Time (PT) and Activated Partial Thromboplastin Time (aPTT) were used to assess the anticoagulant activity of human blood exposed to Heparin, Normal Saline Solution (NSS), and Ginger crude leaf extract. Results showed that blood treated with Heparin had the most prolonged mean PT and aPTT, 67.66±4.68 and 108.59±6.80 seconds, respectively, followed by blood treated with Ginger crude leaf extract, 24.91±4.68 and 58.43±8.50 seconds, respectively. The mean PT and aPTT clotting time of blood treated with NSS were within the normal range, 13.82±0.66 and 33.32±1.42 seconds, respectively. One-way ANOVA showed that the mean PT and aPTT were significantly affected by the type of treatment group, F (1.356, 18.977) = 721.861, p < 0.5, η2 = 98.1% and F(1.391, 19.474) = 510.060, p < .05,η2 = 97.3%. Bonferroni post-hoc tests showed that the PT and aPTT had significant differences between treatment pairs. With these results, we concluded that the 250 mg/mL of Zingiber officinale (Ginger) crude leaf extract has anti-coagulant activity on human blood.
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Copyright (c) 2023 Dave Linconada, Deborah Micah Bereso, Christine Aumella Cale, Hyde Jozel P. Cartoneros, Jean Agnes T. Diu, Norabelle V. Nuñez, Maylyn Grace A. Vallega, Cherish Marie B. Songco
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