The Gastroprotective Activity of Citrullus lanatus (Watermelon) Rind Flavonoid Extract on Aspirin- Induced Gastric Acid Secretion In Male Sprague-Dawley Rats (Rattus norvegicus)

Authors

  • Jan Harmony Cesar Cebu Doctors' University
  • Kim Marie Pearl S. Joe Cebu Doctors' University
  • Tessejim M. Alima Cebu Doctors' University
  • Eric John G. Canangca-an Cebu Doctors' University
  • Risen John S. Delos Reyes Cebu Doctors' University
  • Amanda Paige T. Winrock Cebu Doctors' University

Keywords:

Gastroprotective activity, flavonoid, watermelon, gastric acid secretion

Abstract

This study determines the potential gastroprotective activity of the watermelon rind flavonoid extract by measuring the volume of gastric acid secreted by Sprague-Dawley rats. Twenty-one rats were utilized in the study and these were randomly assigned into three groups. The rats were weighed and their individual weights were used to calculate the dose of flavonoid extract, aspirin suspension, and omeprazole suspension to be administered. Aspirin suspension was used to induce gastric acid secretion in all three groups. Omeprazole suspension was given to the positive control group, distilled water to the negative control group, and flavonoid extract to the experimental group. After 1 hour, all rats underwent cervical dislocation and their gastric volumes were measured. The experimental group had the lowest mean gastric volume (0.19 mL), the positive control group came second (0.20 mL), and the negative control group had the highest mean gastric volume (0.67 mL). The flavonoid extract had the greatest effect in decreasing the gastric acid secretion of the test animals. The positive control group and experimental group showed no significant difference in gastric acid secretion (p value = 1). This shows that watermelon rind flavonoid extract exhibits an effect on gastric acid secretion similar to that of omeprazole.

References

Aktar, M. W., Sengupta, D., & Chowdhury, A. (2009). Impact of pesticides use in agriculture: Their benefits and hazards. Interdisciplinary Toxicology. https://doi.org/10.2478/v10102-009-0001-7

Cleveland Clinic. (2022). Peptic ulcer disease. https://my.clevelandclinic.org/health/diseases/10350-peptic-ulcer-disease

Kolawole, T. A., Dapper, D. V., Oluwatayo, O. B., & Wali, C. (2016). Effects of methanolic extract of the rind of Citrullus lanatus (watermelon) in aspirin-induced gastric ulceration in male Wistar rats. Merit Research Journal of Medicine and Medical Sciences, 4(7), 344-350. v

Lin, W., Lin, M., Zhou, H., Wu, H., Li, Z., & Lin, W. The effects of chemical and organic fertilizer usage on rhizosphere soil in tea orchard. PLoS One. https://doi.org/10.1371/journal.pone.0217018

Legido-Quigley, H. & Asgari-Jirhandeh, N. (‎2018)‎. Resilient and people-centred health systems: Progress, challenges and future directions in Asia. World Health Organization. Regional Office for South-East Asia. https://apps.who.int/iris/handle/10665/276045.

Malik, T. F., Gnanapandithan, K., & Singh, K. Peptic ulcer disease. Retrieved: https://www.ncbi.nlm.nih.gov/books/NBK534792/

Mondal, A. (2011). Advanced plant taxonomy. New Central Agency.

Mota, K. S., Dias, G. E., Pinto, M. E., Luiz-Ferreira, A., Souza-Brito, A. R., Hiruma-Lima, C. A., Barbosa-Filho, J. M., & Batista, L. M. (2009). Flavonoids with gastroprotective activity. Molecules, 14(3), 979-1012. https://doi.org/10.3390/molecules14030979

Nijveldt, R., Nood, E., Hoorn, D., Boelens, P., Norren, K., &Leeuwen, P. (2001). Flavonoids: a review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutrition 104(3), 418-425. https://doi.org/10.1093/ajcn/74.4.418

Oteng Mintah, S., Asafo-Agyei, T., Archer, M.-A., Atta-Adjei Junior, P., Boamah, D., Kumadoh, D., … Agyare, C. (2019). Medicinal Plants for Treatment of Prevalent Diseases. Pharmacognosy - Medicinal Plants. https://doi.org/10.5772/intechopen.82049

Quisumbing, E. (1978). Medicinal plants of the Philippines. Katha Publishing

Rabiu, S., & Muhammad, S. A. (2015). Antiulcerogenic effect of aqueous seed and rind extracts of Citrullus lanatus (watermelon) in ethanol-induced gastric ulcer albino rats. Nigerian Journal of Basic and Applied Sciences, 23(2), 111-115. http://www.scopemed.org/?jft=97&ft=97-1461053713

The Editors of Encyclopedia Britannica. (n.d.). Peptic ulcer. In Encyclopedia Britannica. Retrieved June 6, 2022 from https://www.britannica.com/science/peptic-ulcer

World Health Rankings. (2020). Philippines: Peptic ulcer disease. https://www.worldlifeexpectancy.com/philippines-peptic-ulcer-disease

Zayachkivska, O. S., Konturek, S. J., Drozdowicz, D., Konturek, P. C., Brozowski, T., Ghegotshy, M. R. (2005). Gastroprotective effects of flavonoids in plant extracts. Journal of Physiology and Pharmacology, 56, 2129-231. http://www.jpp.krakow.pl/journal/archive/03_05_s1/pdf/219_03_05_s1_article.pdf

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Published

2022-10-18

How to Cite

Cesar, J. H., Joe, K. M. P., Alima, T., Canangca-an, E. J., Delos Reyes, R. J., & Winrock, A. P. (2022). The Gastroprotective Activity of Citrullus lanatus (Watermelon) Rind Flavonoid Extract on Aspirin- Induced Gastric Acid Secretion In Male Sprague-Dawley Rats (Rattus norvegicus). Journal of Scientific Investigations, 1(1), 21–27. Retrieved from https://jsi.cebudoctorsuniversity.edu/index.php/jsi/article/view/45

Issue

Vol. 1 No. 1 (2022)

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Articles

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