In Vitro Anticoagulant Activity of Zingiber officinale (Ginger) Leaf Extract on Human Whole Blood Using Prothrombin Time and Activated Partial Thromboplastin Time

Authors

  • Dave Linconada Cebu Doctors' University
  • Deborah Micah Bereso Cebu Doctors' University
  • Christine Aumella Cale Cebu Doctors' University
  • Hyde Jozel P. Cartoneros Cebu Doctors' University
  • Jean Agnes T. Diu Cebu Doctors' University
  • Norabelle V. Nuñez Cebu Doctors' University
  • Maylyn Grace A. Vallega Cebu Doctors' University
  • Cherish Marie B. Songco Cebu Doctors' University

Keywords:

anticoagulant activity, human whole blood, partial thromboplastin time, prothrombin time, Zingiber officinale (Ginger) crude leaf extract

Abstract

This study aims to determine the in vitro anticoagulant activity of Zingiber officinale (Ginger) crude leaf extract on human whole blood. Prothrombin Time (PT) and Activated Partial Thromboplastin Time (aPTT) were used to assess the anticoagulant activity of human blood exposed to Heparin, Normal Saline Solution (NSS), and Ginger crude leaf extract. Results showed that blood treated with Heparin had the most prolonged mean PT and aPTT, 67.66±4.68 and 108.59±6.80 seconds, respectively, followed by blood treated with Ginger crude leaf extract, 24.91±4.68 and 58.43±8.50 seconds, respectively. The mean PT and aPTT clotting time of blood treated with NSS were within the normal range, 13.82±0.66 and 33.32±1.42 seconds, respectively. One-way ANOVA showed that the mean PT and aPTT were significantly affected by the type of treatment group, F (1.356, 18.977) = 721.861, p < 0.5, η2 = 98.1% and F(1.391, 19.474) = 510.060, p < .05,η2 = 97.3%. Bonferroni post-hoc tests showed that the PT and aPTT had significant differences between treatment pairs. With these results, we concluded that the 250 mg/mL of Zingiber officinale (Ginger) crude leaf extract has anti-coagulant activity on human blood. 

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Published

2023-12-15

How to Cite

Linconada, D., Bereso, D. M., Cale, C. A., Cartoneros, H. J., Diu, J. A., Nuñez, N., Vallega, M. G., & Songco, C. M. (2023). In Vitro Anticoagulant Activity of Zingiber officinale (Ginger) Leaf Extract on Human Whole Blood Using Prothrombin Time and Activated Partial Thromboplastin Time. Journal of Scientific Investigations, 2(2), 50–56. Retrieved from https://jsi.cebudoctorsuniversity.edu/index.php/jsi/article/view/49

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