In Vitro Anticoagulant Activity of Zingiber officinale (Ginger) Leaf Extract on Human Whole Blood Using Prothrombin Time and Activated Partial Thromboplastin Time


  • Dave Linconada Cebu Doctors' University
  • Deborah Micah Bereso Cebu Doctors' University
  • Christine Aumella Cale Cebu Doctors' University
  • Hyde Jozel P. Cartoneros Cebu Doctors' University
  • Jean Agnes T. Diu Cebu Doctors' University
  • Norabelle V. Nuñez Cebu Doctors' University
  • Maylyn Grace A. Vallega Cebu Doctors' University
  • Cherish Marie B. Songco Cebu Doctors' University


anticoagulant activity, human whole blood, partial thromboplastin time, prothrombin time, Zingiber officinale (Ginger) crude leaf extract


This study aims to determine the in vitro anticoagulant activity of Zingiber officinale (Ginger) crude leaf extract on human whole blood. Prothrombin Time (PT) and Activated Partial Thromboplastin Time (aPTT) were used to assess the anticoagulant activity of human blood exposed to Heparin, Normal Saline Solution (NSS), and Ginger crude leaf extract. Results showed that blood treated with Heparin had the most prolonged mean PT and aPTT, 67.66±4.68 and 108.59±6.80 seconds, respectively, followed by blood treated with Ginger crude leaf extract, 24.91±4.68 and 58.43±8.50 seconds, respectively. The mean PT and aPTT clotting time of blood treated with NSS were within the normal range, 13.82±0.66 and 33.32±1.42 seconds, respectively. One-way ANOVA showed that the mean PT and aPTT were significantly affected by the type of treatment group, F (1.356, 18.977) = 721.861, p < 0.5, η2 = 98.1% and F(1.391, 19.474) = 510.060, p < .05,η2 = 97.3%. Bonferroni post-hoc tests showed that the PT and aPTT had significant differences between treatment pairs. With these results, we concluded that the 250 mg/mL of Zingiber officinale (Ginger) crude leaf extract has anti-coagulant activity on human blood. 


Akbari, A., Nasiri, K., & Heydari, M. (2020). Ginger (Zingiber officinale Roscoe) extract can improve the levels of some trace elements and total homocysteine and prevent oxidative damage induced by ethanol in rat eye. Avicenna Journal of Phytomedicine, 10(4), 365.

El-Hameid, A., Asmaa, R., Abo El-kheir, Z. A., Abdel-Hady, M. S., & Helmy, W. A. (2020). Identification of DNA variation in callus derived from Zingiber Officinale and anticoagulation activities of Ginger Rhizome and callus. Bulletin of the National Research Centre, 44(1), 1-8.


Fu, X., Wang, J., Jiang, S., Wu, J., Mu, Z., Tang, Y., Wang, S., Fu, H., Ma, H., & Zhao, Y. (2023). Mortality trend analysis of ischemic heart disease in China between 2010-2019: A joinpoint analysis. BMC Public Health, (23), 644.

HORIBA Medical. (2021, January 27). Instructions for use: Yumizen G PT Liq 4 Prothrombin Time reagent.

HORIBA Medical. (2021, January 26). Instructions for use: Yumizen G APTT Liq 2 Activated Partial Thromboplastin Time reagent.

Ilkhanizadeh, B., Shirpoor, A., Ansari, M.H.K., Nemati, S., & Rasmi, Y. (2016). Protective effects of ginger (Zingiber officinale) extract against diabetes-induced heart abnormality in rats. Diabetes & Metabolism Journal, 40(1), 46-53.

Patriarcheas, V., Pikoulas, A., Kostis, M., Charpidou, A., & Evangelos, D. (2020). Heparin-induced thrombocytopenia: Pathophysiology, diagnosis and management. Cureus, 12(3).

Shadrack, K., Faraj, A., Alex, M. K., & Kenneth, N. (2019). Anti-thrombotic effect of Zingiber officinale (Ginger) in sprague dawley rats. International Journal of Research in Medical Sciences, 7(9), 3239–3245.

Shukar, S., Zahoor, F., Hayat, K., Saeed, A., Gillani, A.H., Omer, S., Hu, S., Babar, Z.U.D., Fang, Y., & Tang, C. (2021). Drug shortage: Causes, impact and mitigation strategies. Frontiers in Pharmacology, 21. https://doi.org10.3389/fphar.2021.693426

Taj, S., Kashif, A., Fatima, S. A., Imran, S., Lone, A. & Ahmed, Q. (2021). Role of hematological parameters in the stratification of COVID-19 disease severity. Annals of Medicine & Surgery, 62, 68-72. Elsevier.

Tzeng, T.-F., Liou, S.-S., Tzeng, Y.-C., & Liu, I-M. (2016). Zerumbone, a phtochemical of subtropical ginger, protects against hyperglycemia-induced retinal damage in experimental diabetic rats. Nutrients, 8(8), 449.

World Health Organizations. (2021, June 11). Cardiovascular diseases (CVDs).

World Health Organization. (2012, January 1). Blood donor selection: Guidelines on assessing donor suitability for blood donation.

Yee, J. (2020, December 13). Heart Disease in PH worst in Southeast Asia-WHO.




How to Cite

Linconada, D., Bereso, D. M., Cale, C. A., Cartoneros, H. J., Diu, J. A., Nuñez, N., Vallega, M. G., & Songco, C. M. (2023). In Vitro Anticoagulant Activity of Zingiber officinale (Ginger) Leaf Extract on Human Whole Blood Using Prothrombin Time and Activated Partial Thromboplastin Time. Journal of Scientific Investigations, 2(2), 50–56. Retrieved from