In Vitro Anticoagulant Activity of Zingiber officinale (Ginger) Leaf Extract on Human Whole Blood Using Prothrombin Time and Activated Partial Thromboplastin Time
Keywords:
anticoagulant activity, human whole blood, partial thromboplastin time, prothrombin time, Zingiber officinale (Ginger) crude leaf extractAbstract
This study aims to determine the in vitro anticoagulant activity of Zingiber officinale (Ginger) crude leaf extract on human whole blood. Prothrombin Time (PT) and Activated Partial Thromboplastin Time (aPTT) were used to assess the anticoagulant activity of human blood exposed to Heparin, Normal Saline Solution (NSS), and Ginger crude leaf extract. Results showed that blood treated with Heparin had the most prolonged mean PT and aPTT, 67.66±4.68 and 108.59±6.80 seconds, respectively, followed by blood treated with Ginger crude leaf extract, 24.91±4.68 and 58.43±8.50 seconds, respectively. The mean PT and aPTT clotting time of blood treated with NSS were within the normal range, 13.82±0.66 and 33.32±1.42 seconds, respectively. One-way ANOVA showed that the mean PT and aPTT were significantly affected by the type of treatment group, F (1.356, 18.977) = 721.861, p < 0.5, η2 = 98.1% and F(1.391, 19.474) = 510.060, p < .05,η2 = 97.3%. Bonferroni post-hoc tests showed that the PT and aPTT had significant differences between treatment pairs. With these results, we concluded that the 250 mg/mL of Zingiber officinale (Ginger) crude leaf extract has anti-coagulant activity on human blood.
References
Akbari, A., Nasiri, K., & Heydari, M. (2020). Ginger (Zingiber officinale Roscoe) extract can improve the levels of some trace elements and total homocysteine and prevent oxidative damage induced by ethanol in rat eye. Avicenna Journal of Phytomedicine, 10(4), 365. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430965/pdf/AJP-10-365.pdf
El-Hameid, A., Asmaa, R., Abo El-kheir, Z. A., Abdel-Hady, M. S., & Helmy, W. A. (2020). Identification of DNA variation in callus derived from Zingiber Officinale and anticoagulation activities of Ginger Rhizome and callus. Bulletin of the National Research Centre, 44(1), 1-8. https://doi.org/10.1186/s42269
-020-0281-9
Fu, X., Wang, J., Jiang, S., Wu, J., Mu, Z., Tang, Y., Wang, S., Fu, H., Ma, H., & Zhao, Y. (2023). Mortality trend analysis of ischemic heart disease in China between 2010-2019: A joinpoint analysis. BMC Public Health, (23), 644. https://doi.org/10.1186/s12889-023-15549-3
HORIBA Medical. (2021, January 27). Instructions for use: Yumizen G PT Liq 4 Prothrombin Time reagent. https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&ved=2ahUKEwicxKbc2JODAxXarVYBHZCbCHQQFnoECAoQAQ&url=https%3A%2F%2Ftoolkits.horiba-abx.com%2Fdocumentation%2Fdownload.php%3Fid%3D341460&usg=AOvVaw0ASE9o-phH8-EW4HlBRpH2&opi=89978449
HORIBA Medical. (2021, January 26). Instructions for use: Yumizen G APTT Liq 2 Activated Partial Thromboplastin Time reagent. https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwicxKbc2JODAxXarVYBHZCbCHQQFnoECAkQAQ&url=https%3A%2F%2Ftoolkits.horiba-abx.com%2Fdocumentation%2Fdownload.php%3Fid%3D349540&usg=AOvVaw0ivAylcbfyG1iqLkyZgldW&opi=89978449
Ilkhanizadeh, B., Shirpoor, A., Ansari, M.H.K., Nemati, S., & Rasmi, Y. (2016). Protective effects of ginger (Zingiber officinale) extract against diabetes-induced heart abnormality in rats. Diabetes & Metabolism Journal, 40(1), 46-53. https://doi.org/10.4093/dmj.2016.40.1.46
Patriarcheas, V., Pikoulas, A., Kostis, M., Charpidou, A., & Evangelos, D. (2020). Heparin-induced thrombocytopenia: Pathophysiology, diagnosis and management. Cureus, 12(3). https://doi.org/10.7759/cureus.7385
Shadrack, K., Faraj, A., Alex, M. K., & Kenneth, N. (2019). Anti-thrombotic effect of Zingiber officinale (Ginger) in sprague dawley rats. International Journal of Research in Medical Sciences, 7(9), 3239–3245. https://doi.org/10.18203/2320-6012.ijrms20193905
Shukar, S., Zahoor, F., Hayat, K., Saeed, A., Gillani, A.H., Omer, S., Hu, S., Babar, Z.U.D., Fang, Y., & Tang, C. (2021). Drug shortage: Causes, impact and mitigation strategies. Frontiers in Pharmacology, 21. https://doi.org10.3389/fphar.2021.693426
Taj, S., Kashif, A., Fatima, S. A., Imran, S., Lone, A. & Ahmed, Q. (2021). Role of hematological parameters in the stratification of COVID-19 disease severity. Annals of Medicine & Surgery, 62, 68-72. Elsevier. https://doi.org/10.1016/j.amsu.2020.12.035
Tzeng, T.-F., Liou, S.-S., Tzeng, Y.-C., & Liu, I-M. (2016). Zerumbone, a phtochemical of subtropical ginger, protects against hyperglycemia-induced retinal damage in experimental diabetic rats. Nutrients, 8(8), 449. https://doi.org/10.3390/nu8080449
World Health Organizations. (2021, June 11). Cardiovascular diseases (CVDs).https://www.who.int/en/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds)
World Health Organization. (2012, January 1). Blood donor selection: Guidelines on assessing donor suitability for blood donation. https://www.who.int/publications/i/item/9789241548519
Yee, J. (2020, December 13). Heart Disease in PH worst in Southeast Asia-WHO. Inquirer.net. https://newsinfo.inquirer.net/1371108/heart-disease-in-ph-worst-in-sea
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Copyright (c) 2023 Dave Linconada, Deborah Micah Bereso, Christine Aumella Cale, Hyde Jozel P. Cartoneros, Jean Agnes T. Diu, Norabelle V. Nuñez, Maylyn Grace A. Vallega, Cherish Marie B. Songco
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