In Vitro Thrombolytic Activity of the Ethanolic Fruit Pulp Extract of Cresentia cujete (Calabash) on Human Whole Blood


  • Cherish Songco Cebu Doctors' University
  • Wilfred C. Cai Cebu Doctors' University
  • Shara Khris D. Lanugan Cebu Doctors' University
  • Cesar D. Misador Jr. Cebu Doctors' University
  • Khayla Ray Bernadine M. Rondobio Cebu Doctors' University
  • Kent O. Saarenas Cebu Doctors' University
  • Chytel Marie N. Sun Cebu Doctors' University
  • Dorothy Mariz Z. Tan Cebu Doctors' University
  • Bhea Jessa N. Zerda Cebu Doctors' University


Crescentia cujete (Calabash) thrombolytic activity, streptokinase, in vitro thrombolytic activity


Cardiovascular diseases (CVD) are a prominent cause for mortality worldwide. These conditions often increase the risk of blood clot formation (thrombosis) and may result in death. Due to the financial difficulties of people from lower income countries such as the Philippines, patients have difficulty in complying with the expensive medications prescribed to be taken and opt for alternative remedies from among locally available resources. This study aims to identify the in vitro thrombolytic activity of the ethanolic fruit pulp extract of Crescentia cujete (calabash) on human whole blood. The Streptokinase solution (positive control) showed a mean absorbance of 1.22±0.790. However, the two concentrations of the Crescentia cujete ethanolic fruit pulp extract, namely, 35 mg/mL and 17.5 mg/mL, showed a mean absorbance of 0.980±0.752 and 0.787±0.818, respectively. The normal saline solution (negative control) showed a mean absorbance of 0.716±0.885. The results of one-way repeated measures ANOVA showed that the absorbance of hemoglobin released from clotted blood was significantly different among the treatment and control groups with F (2.149, 40.825) = 20.541, p < .05, n2 = 51.9%. Analysis of the results show that the 35 mg/mL ethanolic fruit pulp extract of Crescentia cujete has thrombolytic activity on human whole blood.


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How to Cite

Songco, C., Cai, W., Lanugan, S. K., Misador , C., Rondobio, K. R. B., Saarenas, K., Sun, C. M., Tan, D. M., & Zerda, B. J. (2023). In Vitro Thrombolytic Activity of the Ethanolic Fruit Pulp Extract of Cresentia cujete (Calabash) on Human Whole Blood. Journal of Scientific Investigations, 2(2), 27–34. Retrieved from